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20 μm protein and 200 μm ANS in PBS were used for ANS fluorescence measurement for each protein sample and data were recorded on Fluoromax-4 (Horiba Jobin Yvon) spectrofluorometer. Samples were excited at 380 nm (Slit width 5 nm), and emission spectra were recorded from 385 to 650 nm (slit width 10 nm) at 25 °C. Averages of three independent scans are reported in Fig. 2d.
Copyright and License information: ©2019 Chatterjee et al.
Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.
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