ELISA assay for cytokines

JP Jin-Young Park
ML Man-Sup Lim
SK Song-In Kim
HL Hee Jae Lee
SK Sung-Soo Kim
YK Yong-Soo Kwon
WC Wanjoo Chun
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The RAW264.7 macrophage cells were treated with QG in the absence or presence of LPS. After 24 hr incubation, TNF-α and IL-1β levels in culture media were quantified using monoclonal anti-TNF-α or IL-1β antibodies according to the manufacturer’s instruction (R&D Systems, Minneapolis, MN, USA).

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