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The ISceI-based DR-GFP reporter assay was performed in cells following IB treatment (1, 2.5 or 5 μM for 3, 12 or 24 hours) to evaluate frequency of double strand break repair by homologous recombination as described previously (18-20). For rescue experiments, cells were pre-treated with IB or vehicle control for 3 hours followed by transfection with control, FoxM1 expression plasmid and ISCeI expression vector respectively for 48 hours.
Copyright and License information: ©2016
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