Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Monosodium urate (MSU) induced peritonitis model

GH Guillaume Hanouna
LM Laurent Mesnard
SV Sophie Vandermeersch
JP Joëlle Perez
SP Sandrine Placier
JH Jean-Philippe Haymann
FC Fabien Campagne
JM Julien Moroch
AB Aurélien Bataille
LB Laurent Baud
EL Emmanuel Letavernier
request Request a Protocol
ask Ask a question
Favorite

MSU crystals were prepared with 500 ml of boiling water and 2 g of uric acid (U2625, Sigma Aldrich). pH solution was maintained at 8 by adding NaOH (1 M). Solution was cooled and kept 24 hours during crystals formation. Crystals were filtered in 100 µm sieve before washing in ethanol and warm sterilization. Peritonitis was induced in 8-weeks old WT and CalpTG females by intraperitoneal (I.P.) injection of 3 mg MSU diluted in 0.5 ml PBS. After 3 hours, peritoneal cavity was flushed with 3 ml PBS heparin for 3 mn. Peritoneal fluid was centrifugated at 4 °C and supernatant has been analyzed by ELISA for Interleukine 1 α (Il-1α) and β (Il-1β) (MLA00, MLB00C, R&D systems).

Copyright and License information: The Author(s) ©2017
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A