Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

4.3. Cell Viability

KK Kaja Karaś
AS Anna Sałkowska
IK Iwona Karwaciak
AW Aurelia Walczak-Drzewiecka
JD Jarosław Dastych
RB Rafał A. Bachorz
MR Marcin Ratajewski
request Request a Protocol
ask Ask a question
Favorite

The cytotoxicity of AZ5104 in HepG2 cells was evaluated with the neutral red uptake assay [81]. The absorbance of each sample was determined spectrophotometrically at 550 nm using a Sunrise microplate reader (Tecan, Männedorf, Switzerland). The cytotoxicity of AZ5104 in Th17 cells was determined using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Fitchburg, WI, USA) according to the manufacturer’s protocol. The luminescence of each sample was determined with an Infinite® 200 PRO (Tecan, Männedorf, Switzerland).

Copyright and License information:  ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A