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2.4. Diet induced obesity mouse study

MT Matteo Tardelli
KZ Karina Zeyda
VM Veronica Moreno-Viedma
BW Bettina Wanko
NG Nicole G. Grün
GS Günther Staffler
MZ Maximilian Zeyda
TS Thomas M. Stulnig
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Eight male WT (C57BL/6J) and eight male OPN-knockout mice (SPP1KO; B6.Cg-Spp1tm1Blh/J) were purchased by Charles River (Sulzfeld, Germany) and fed a high-fat diet (HFD, 60 kcal %, D12492; Research Diets, New Brunswick, NJ, USA) for 8 or 12 weeks. After the indicated time, mice were sacrificed and gonadal white adipose tissue (GWAT) was collected. The protocol was approved by the local ethics committee for animal studies.

Copyright and License information:  ©2016 The Author(s)
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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