3.8. Ovariectomized Osteoporosis Model Mice

The OVX and sham-operated eight-week-old female ddY mice were purchased from Shizuoka Laboratory Center Inc. (Hamamatsu, Japan). The mice were acclimated for 10 days prior to experimentation. The mice were maintained on a diet (5.0 g/day) of Formula-M07 (Feedlab Co. Ltd.; Hanam, Korea) and tap water (15 ml/day). All mice were housed individually in clear plastic cages under controlled temperature (23 ± 2 °C), humidity (55 ± 5%), and illumination (12-hour light/dark cycle). Mice were orally administered KB (2 or 5 mg/kg/day) for 12 weeks. The BMD and BMC were measured at 0, 6, and 12 weeks during the experiment. The animal research procedures were approved by the Animal Care and Use Committee of the Ajou University School of Medicine (IACUC No. 2014-0066, approved at 23 September 2015), and all experiments were conducted in accordance with the institutional guidelines established by the committee. All efforts were made to minimize animal suffering and to reduce the number of mice used.