2.4. Cell Viability Assay

AML cells were seeded in triplicate wells in flat-bottomed 96-well plates at a density of 3 × 10⁵ cells/mL (final volume of 100 µL) and incubated overnight. Γ-tocotrienol was added onto the cells and incubated for 24 or 48 h. At each time point, cell viability was evaluated using an MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt) assay kit (Promega) according to the manufacturer’s instructions. MTS reagent was added to the wells and optical density (OD) values of absorbance were measured at 490 nm using a Varioskan Flash plate reader (Thermo Fisher Scientific). The percentage of proliferating cells was determined relative to 100 percent viability of control untreated cells.