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ATP content of hippocampi and platelets
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ATP contents in freshly prepared hippocampal and platelet homogenates were quantified using the ATP Bioluminescent Assay Kit (Biyutian, China). The reagents and reaction mixture were combined according to the supplied protocol using reversed-phase high-performance liquid chromatography (HPLC) with a calibrated ATP standard curve. Samples were monitored in triplicate. The ATP concentration was calculated as nmol ATP/mg protein, and then normalized by the control group (%).
Copyright and License information: ©2019 Chen et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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