Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Reactive oxygen species (ROS) production on differentiated 3 T3-L1 adipocytes with or without sample treatment was measured using NBT assay. NBT assay was carried as the previously described method with some modification [24]. At Day 8 of MDI induction, 3 T3-L1 cells were incubated for 90 min in PBS containing 0.2% NBT. After washing with PBS, the stained cell plates were scanned. Thereafter, formazan, the resulted product formed by the reduction of NBT with ROS, was dissolved in DMSO, and the absorbance was measured at 520 nm using a microplate reader.
Copyright and License information: The Author(s). ©2019
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
