4.2. Clonogenic Survival and MTT Assay

Cells were seeded to 60–70% confluence in 10 cm plates and treated for the indicated times. 24 h following treatment, cells were washed, serially diluted, and plated in triplicate on 6-well plates (500–1000 cells/well). 6–14 days of cell growth were allowed for colony formation, followed by staining with 0.5% crystal violet. Cell counts were normalized to untreated controls as previously described [47]. Combination treatment scheme is as followed: Co-administration (‘Co-ad’) with both 100 nM tamoxifen (Tamox) and 1 nM talazoparib (Talaz) on day 1, and 100 nM Tamox on days 2 and 3, unless otherwise indicated.