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Chromatin immunoprecipitation (ChIP) assay
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The ChIP assay was performed as described previously 39. The soluble chromatin was incubated with 2 μg of non-specific control IgG, EZH2, H3K27me3 or H3K27ac antibodies. PCR was performed using specific primers for the EZH2 binding region in the FOXO1 promoter. Quantitative real-time PCR was performed with ChIP samples using the iQ SYBR Green Supermix and an iCycler iQTM detection system (Bio-Rad) according to manufacturer's instructions. The primer sequences are listed in Table S2.
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