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Metalation of CobWs was performed after the production of the apoproteins by adding a 10-fold excess of EDTA and incubating for 2 h on ice, and metal-EDTA complexes were removed over a PD10 column (the procedure was modified from that in reference 42). Success was verified by ICP-MS. The metalation was also performed with a 10-fold excess of ZnCl2 with or without GTP plus MgCl2 and subsequent removal of surplus zinc.
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