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3.7. Measurement of Pro-Inflammatory Cytokine Production
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A cytokine assay was conducted to examine the inhibitory action of compounds 1–4 on pro-inflammatory cytokines (IL-6, and TNF-α) as previously described. A mouse enzyme-linked immunosorbent assay (ELISA) kit (NeoBioscience Technology Co, Ltd. Shenzhen, China; Batch No M190726-004a, M190726-102a) was used to measure the proinflammatory cytokine production of supernatants from LPS (100 ng/mL) treated RAW264.7 macrophages.
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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