4.7. AAV Infection and Luciferase Reporter Assays

Recombinant AAV vectors carrying a luciferase expression cassette were used to evaluate the viral transduction efficiency. HeLa cells were seeded in 24-well plates at 80% confluence (about 5 × 10⁵ cells per well). Purified AAV-Luc was used to infect the cells at desired m.o.i. for 24 h. The luciferase activities were measured using a luciferase assay system according to the manufacturer’s protocol (Promega, Madison, WI, USA). A luminometer of MiNiCHEMI (Sagecreation, Beijing, China) was used for the quantitative detection of the luminescence.