Quantitative real-time PCR (qRT-PCR)

Total RNAs were extracted from brain tissues or MN9D cells or primary mesencephalic neurons using Trizol reagent (ThermoFisher Scientific, CA, USA), and inversely transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, CA, USA). Quantitative real-time PCR was performed to measure SNHG14, miR-133b and α-syn expressions using SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (ThermoFisher Scientific, CA, USA). The relative expression of SNHG14, miR-133b and α-syn were expressed as a function of threshold cycle (Ct) and analyzed by 2^-ΔΔCt method.