miRNA microarray analysis and miRNA target prediction.

ZT Zhongzhong Tu
MX Mengxian Xu
JZ Jian Zhang
YF Ye Feng
ZH Zhuo Hao
CT Changchun Tu
YL Yan Liu
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RNAs were extracted from BHK-21 cells infected with CVS-11 at 48 h postinfection (p.i.) using TRIzol reagent (Invitrogen, USA). A miRNA microarray assay was performed by BioClouds (Shanghai, China). The miRNA array data can be accessed on the NCBI’s Gene Expression Omnibus (GEO) database (GSE134156). The miRNA targets in host cells were predicted by the use of the programs TargetScan (version 3.1; Whitehead Institute for Biomedical Research [http://www.targetscan.org/mamm_31/]) and miRDB (http://www.mirdb.org/).

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