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5.4. Determination of Entrapment Efficiency of PTB and Pomace Extract

GS Giovanna Simonetti
CP Cleofe Palocci
AV Alessio Valletta
OK Olga Kolesova
LC Laura Chronopoulou
LD Livia Donati
AN Antonio Di Nitto
EB Elisa Brasili
PT Pierpaolo Tomai
AG Alessandra Gentili
GP Gabriella Pasqua
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The quantitative analysis of PTB entrapped within PLGA NPs was carried out by spectroscopic measurements. NPs aqueous suspensions were ultra-centrifuged at low temperature to recover NPs. NPs were dissolved in acetone and analyzed by measuring the UV absorbance at 283 nm and comparing the results with a calibration curve. To verify the entrapment efficiency of the pomace extract in PLGA NPs, an analysis of the pomace extract before and after its entrapment in PLGA NPs was carried out by HPLC-ESI-MS spectrometry. The entrapment efficiency in PLGA NPs was calculated as percent ratio of the area of grape pomace extract entrapped to the residual amounts of non-entrapped extract.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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