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The free concentrations of piperacillin in sera were assessed using UPLC preceded by ultra-filtration (UHPLC, Agilent 1290, Agilent Technologies, USA), described in detail previously.25,26 Intra-run (total) imprecisions (coefficient of variation) were 10.2% (15.3%) at 4.5 mg/L and 4.7% (8.2%) at 15.6 mg/L. The lower limit of quantification (LLOQ) was 0.5 mg/L.
Copyright and License information: ©2019 The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
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