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Lipid peroxidation was determined by assaying thiobarbituric acid-reactive substances (TBARS) according to the method of Preuss [45]. Briefly, 1.0 ml supernatant was precipitated with 2 ml 7.5 % trichloroacetic acid and centrifuged at 1000g for 10 min. Clear supernatant was mixed with 1 ml 0.70 % thiobarbituric acid, incubated at 80 °C and the absorbance measured at 532 nm. Tetramethoxypropane was used as the standard.
Copyright and License information: Maodaa et al. ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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