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The ATP levels were measured in activated macrophages in the presence or absence of MCU inhibitor, KB-R7943, and after siRNA-mediated MCU silencing. To perform a direct comparison, cells were harvested and counted, and the same number of cells were re-suspended in 100 µL. The ATPlite assay was performed according to the manufacturer’s instructions (PerkinElmer, Waltham, MA, USA). Luminescence was measured with a FLUOstar OPTIMA luminescence plate reader (BMG Labtech, Ortenberg, Germany).
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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