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A549 cells were seeded on coverslips and transfected with MAPKAPK5-AS1 siRNA or control siRNA. After 48 h, cells were fixed and incubated with Ki-67 antibody (Cell Signaling Technology, Danvers, MA, USA) for 1 h and then incubated with Alexa-488 at room temperature for 20 min. Cells were then counterstained with DAPI to detect the cell nuclei. All coverslips were mounted using prolong® diamond antifade mountant (ABI, USA.)
Copyright and License information: ©2019 Annals of Translational Medicine. All rights reserved.
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