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Serum Analysis by Size-Exclusion Chromatography.
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Sera were pooled from 4 mice, and 1 mL of the pooled serum was separated by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences). Samples were processed at a flow rate of 0.2 mL/min in PBS with 1.5 mM EDTA. The eluate was collected as 1-mL fractions, snap-frozen in liquid nitrogen, and stored at −80 °C until analysis.
Copyright and License information: ©2019 the Author(s). Published by PNAS.
This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
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