4.6. Quantitative Real Time Polymerase Chain Reaction (qRT-PCR)

To validate the gene expression of each factor, 1 µg of cDNA was used with the primers (Supplementary Table S1.) and distilled water. The solution was added 10 µL of cyber green solution (CYBG, TAKARA, Mountain View, CA, USA). The mixed solution was distributed in a 384-well plate (Bio-RAD, Hercules, CA, USA). CFX386 touch (Bio-Rad, Hercules, California, USA) was used to perform the qRT-PCR. The reaction efficiency and the number of cycles were determined by innate software.