2.5. BrdU Incorporation Assay

Here, five thousand cells/well were seeded into 96-well plates. Cells were then starved for 48 h before commencing any treatment. Cell proliferation was then measured with BrdU kit (Roche Diagnostics, Penzberg, Germany) following the manufacturer’s protocol. Optical density was measured using a microplate reader spectrophotometer at excitation wavelength 450 nm.