PCR primers and probes

Primers and probes used are listed below (F—forward, P—probe, R—reverse). All probes were synthesized by Integrated DNA Technologies and fluorophore used was FAM with two quenchers ZEN and Iowa Black FQ.

F: tgtgtctgaacctgagcctg

P: agcccgagccagaaccggagcctgcaa

R: atagcaggcgccattttagg

F: ttctgctgtgcgtaacttgc

P: tggaacagagctctaacagtacctcttggttttggag

R: actaactttgcctgggatgagc

F: aactgtcacctgctgaagacc

P: acgtagccagccactctcgcaaggcctgg

R: acccaaatgcaaggaacagc

F: accatggtgtcatagtccagc

P: agcttgcccttggaggaggcgccgca

R: agctctacgccctcaaaagtc

F: atcctgcagagcatagtggtg

P: agcctggctgacaaggtggccgcca

R: tcttgcgggcgtaaaacttg

F: tccatggttgcactaaacgc

P: agtacacagcccgccaacgtgccgcg

R: cgctcacaaagttggtgtagtc

F: agcaatgacagcaccttcac

P: acggcgaccctcagaccggaatccgct

R: ttacgtcaggagtgcaaagc

F: agcatttgcctttacgccac

P: tggcccacaacaccgcctccacgctt

R: tgtcgtttctaagcatggcc

F: tttcttcttgggcgcaatgg

P: aggtcgatggccgcgggctgggtgt

R: aagactcatcacaagacgcg

F: tctgcaacaggaaaacagcg

P: agggtgacaacgcgcgcctagccgt

R: acctcgatgctgcgttttag

F: ttgttgccatctctgtgctg

P: acgccaccgtcttcacccgcccaagcaa

R: aaagtaccaggtaaggttcgcc

F: atgcttgcgctcaaaatggg

P: tctggacgaggccggcaaccttacctccca

R: tttttgagaggtgggctcac

F: aagttcatgtcgctgtccag

P: agccacaggctgctgtccaacttgcg

R: gtggacttctccttcgccg

F: gcacaatggcttccaaaagg

P: acggccctcacgtccaagtggacg

R: tttgggcatggttgaaggtg

F: ttttccaagggttgagtcgc

P: tcgagtctcggaccggccggac

R: aatttgcaagcggggtcttg

Real-time PCR primers for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and E4orf3 were previously described [8, 17–20].