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All siRNAs were purchased from Dharmacon. The siRNA sequences are listed in Supplementary Materials and Methods. siRNA (20 μL of 20 μM siRNA/transfection) was mixed with 5–8 × 106 cells in 180 μL media in 4-mm cuvette and electoporated with a BTX ECM 830 electroporator with two, 280-volt, 10-msec pulses).
Copyright and License information: ©2019
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