2.3. Exploration through Semi-Quantitative PCR

The semi-quantitative PCR reaction for characterization of SYCP3 and TSEG2 in different tissues comprised a total of 162.5 µL Taq PCR master mixture, 13 µL each primer (forward and reverse), and ddH₂O 123.5 µL after that reaction mixture were divided into 24 µL aliquots into 11 tubes. Additionally, cDNA obtained from ten kinds of tissues was used, 1 µL of the cDNA was added into the ten tubes, and one tube as a control. The reaction was conducted according to Yan et al. [22]. The PCR was implemented using the different conditions: for 3 min at 94 °C; followed by 30 cycles of 94 °C for 30 s, annealing at 60 °C (for GAPDH, SYCP3 and TSEG2) for 30 s, and 72 °C for 1 min; and extension at 72 °C for 5 min. The reaction products were visualized on 1 % agarose gel stained with ethidium bromide.