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The size of the exosomes and microvesicles was determined by dynamic light scattering (DLS) using a Zetasizer nano ZS90 particle sizer (Malvern Instruments, Worcestershire, UK) at a 90° fixed angle. The particle diameter was calculated using the Stokes–Einstein equation. For particle sizing in solution, exosome and microvesicle aliquots were diluted in 10% PBS and analyzed at a constant 25 °C.
Copyright and License information: ©2019 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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