Reads from the zebra finch, Bengalese finch, Eurasian siskin, and pale martin GRC sequences were aligned to the assembly of the zebra finch genome (Taeniopygia_guttata-3.2.4) using BLAT (21). A custom python script was used to estimate the coverage of the zebra finch genome in 10-kb windows. Overlapping of regions with high coverage with zebra finch Ensembl gene predictions and nonzebra finch RefSeq (23) genes was revealed with the Ensembl genome browser. Repeat content of the GRC libraries and the zebra finch genome was assessed with RepeatMasker (25) by using the avian RepBase database (26).
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