EXPERIMENTAL MODEL AND SUBJECT DETAILS

All the procedures involving mice were carried out in strict accordance with the recommendations in the Eight Edition of the Guide for the Care and Use of Laboratory Animals of the National Research Council of the National Academies. All protocols were approved by Thomas Jefferson University’s Institutional Animal Care and Use Committee. All mice used in experiments were 6–12 weeks old and males and females were both used. Colonies were bred at Thomas Jefferson University under specific pathogen free conditions. Animals were housed in a controlled environment with 12-hour day/night cycle with optimal temperature and fed rodent chow ad libitum. C57BL/6 (B6) mice were purchased from Charles River. B6.129S(C)-Batf3^tm1Kmm/J (Batf3^−/−) mice were purchased from Jackson Laboratories. Breeder mice expressing photoactivatable green fluorescence protein under the mouse Ubiquitin C promoter (Ubc-PA-GFP mice) were a gift from Dr. Gabriel Victora (Rockefeller University, New York, NY). Breeder mice expressing the human diphtheria toxin receptor under the human Langerin (CD207) promoter (CD207-DTR mice) mice were a gift from Dr. Daniel Kaplan (University of Pittsburgh, Pittsburg, PA).