TBUT was measured to assess the tear film stability. Fluorescein was instilled into the subject’s left eye, and the subject was instructed to refrain from blinking while the tear film was observed. A yellow barrier filter was used to enhance the visibility of the tear film break-up. TBUT was recorded as the number of seconds that elapsed between the last blink and the appearance of the first dry spot in the tear film. The measurement was performed in both eyes three times, and the average time was recorded. The cutoff value for dry eye is <10 seconds.37
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