2.2. Intracerebroventricular (ICV) Cannulation

The mice were anesthetized with an intraperitoneal (i.p.) injection of tribromoethanol (250 mg/kg, Sigma-Aldrich, St. Louis, MO, USA) and placed in a stereotaxic apparatus (Stoelting, Wood Dale, IL, USA). The cannula (26 gauge) was implanted into the right lateral ventricle (coordinates: 0.1 mm lateral, 0.03 mm posterior, and 2.4 mm ventral to the bregma) of the mice and secured to their skulls using dental cement. The animals were kept warm until they recovered from the anesthesia and then were placed in individual cages. After surgery, a recovery period of 7 days was allowed before starting the experiments. To evaluate the effects of BAIBA on saturated fatty acid-induced microglial activation in the hypothalamus, palmitic acid (25 pmol/µL) was injected through the cannula after the systematic pre-treatment of the mice with BAIBA (3 mg/kg; i.p. injection).