Detoxified corn cob hydrolysate

To remove inhibitors (phenolic compounds, weak acids and furans), corn cob hydrolysate was detoxified using activated charcoal and ionic exchange resins. Corn cob hydrolysate was mixed with activated charcoal at liquor to solid ratio of 10 g of hydrolysate per gram of activated charcoal with agitation for 1 h [50] to remove phenolic compounds. After that, the hydrolysate was treated with ion exchange resins as described by Rodríguez-López et al. [50]. The hydrolysate was mixed with Amberlite IR-120 resin in H⁺ form at a ratio 10 g of cationic resin per gram of hydrolysate for 1 h with agitation. Cationic resin was recovered by filtration and the hydrolysate was treated for 2 h under agitation with Mto-Dowex M43 (anionic) resin in OH⁻ form at a ratio 20 g of anionic resin per gram of acetic acid present in the hydrolysate. The detoxified hydrolysate was sterilized by filtration and analyzed by HPLC.