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BLAST analysis was carried out using blastp developed by NCBI against the ‘non-redundant protein sequences (nr)’ database, where the matrix BLOSUM62 was used. The gene ontology (GO) annotations and Enzyme Commission numbers were then assigned to the proteins using BLAST2GO plugin of CLC workbench according to the results of BLAST analysis. Fisher’s exact test was applied to compare the distribution of differential proteins with that of all detected proteins in GO categories. As a result, the enriched GO categories were filtered by FDR = 5%.
Copyright and License information: ©2019 Wang, Que, Wang, Hao, Li, Xu and Xiong.
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