Chemical compounds and small-molecule screening

Tg(snai1b:GFP) zebrafish embryos at ∼13 hpf were treated in 12-well plates. 8-10 embryos/well were incubated at 28°C in 1 ml of egg water containing pharmacological inhibitors or 0.1-1% DMSO as control. Pharmacological inhibitors were removed after 6-12 h incubation and embryos screened for EMT and migration defects at 20-24 hpf using fluorescent light on an Olympus SZX16 microscope. TP-0903 (Mollard et al., 2011) was reconstituted to 10 mM in DMSO. All-trans-retinoic acid (RA) and DEAB were purchased from Sigma. Stock solutions of RA (10 mM) and DEAB (10 mM) were prepared in DMSO. All RA treatments were performed in the dark.