Statistical analysis

LX Lei Xiong
FW Feng Wu
QW Qiong Wu
LX Liangliang Xu
OC Otto K. Cheung
WK Wei Kang
MM Myth T. Mok
LS Lemuel L. M. Szeto
CL Cheuk-Yin Lun
RL Raymond W. Lung
JZ Jinglin Zhang
KY Ken H. Yu
SL Sau-Dan Lee
GH Guangcun Huang
CW Chiou-Miin Wang
JL Joseph Liu
ZY Zhuo Yu
DY Dae-Yeul Yu
JC Jian-Liang Chou
WH Wan-Hong Huang
BF Bo Feng
YC Yue-Sun Cheung
PL Paul B. Lai
PT Patrick Tan
NW Nathalie Wong
MC Michael W. Chan
TH Tim H. Huang
KY Kevin Y. Yip
AC Alfred S. Cheng
KT Ka-Fai To
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GraphPad Prism 5 (GraphPad Software) was used for statistical analysis. The difference between two groups was calculated by independent Student’s t-test or Wilcoxon signed-rank test. The correlation among methylation and eRNA/mRNA expression was analyzed using Pearson correlation test. Chi-square test with Yates correction was used to determine whether distributions of H3K27ac levels differ between promoters and enhancers. Kaplan–Meier survival analysis was used to determine the overall and disease-free survival rates, which were calculated from the date of curative surgery to death, HCC recurrence or the last follow-up; the differences were compared by log-rank Mantel–Cox test.

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