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Cells were seeded and treated with the indicated dose of MEK inhibitor (AZD6244, GSK112012, AS703026, or BAY 86-9766) (Selleckchem, Houston, TX). After 24 h, the cells were harvested and evaluated with the Trypan blue exclusion assay. Additionally, DLD-1, HCT-8, DLD-1 isogenic cells (PI3K mutant: 353), and HCT116 isogenic cells (54, 240, and 241) were plated and treated with 5 μM NVP-TNKS656 (tankyrase inhibitor) and 1 µM GSK112012 (MEK inhibitor). After 48 h, the cell death rate was determined with the Trypan blue exclusion assay.
Copyright and License information: Cancer Research UK ©2019
This work is published under the standard license to publish agreement. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution 4.0 International (CC BY 4.0).
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