Genome sequencing and annotation

Aquimarina sp. Aq78 and Aquimarina sp. 349 were retrieved from the marine sponge Sarcotragus spinosulus as described previously.⁵⁶ Prior to whole genome sequencing, genomic DNA was extracted from a pure culture grown in marine broth for five days at 19 °C using the Wizard Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA). Paired-end sequence reads (125 bp) were generated using an Illumina HiSeq2500 platform at BaseClear (Leiden, The Netherlands). FASTQ sequence files were generated using the Illumina Casava pipeline version 1.8.3. Sequencing output was 287 Mb consisting of 2 x 128 bp quality-filtered paired-end reads, resulting in a predicted genome coverage of 48X. Adapter sequences were trimmed and the reads assembled using the CLC Genomics Workbench version 7.0.4. Initial annotation was performed with the RAST (Rapid Annotation using Subsystem Technology) server, version 2.0.⁵⁷ Aquimarina sp. Aq78 shares 98.98% 16S rRNA gene identity with type strain Aquimarina macrocephali JAMB N27, isolated from marine sediment.⁵⁸

The genome sequences of Leptolyngbya sp. PCC 7375 ({"type":"entrez-nucleotide","attrs":{"text":"ALVN00000000","term_id":"425763137","term_text":"ALVN00000000"}}ALVN00000000) and G. sunshinyii YC6258 ({"type":"entrez-nucleotide","attrs":{"text":"NZ_CP007142.1","term_id":"765292753","term_text":"NZ_CP007142.1"}}NZ_CP007142.1) were obtained previously.^36,59