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Quantitative GUS Activity Assay
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ß-Glucuronidase activity of immature seeds (whole seeds, manually separated seed coats and embryos) was estimated as described by Renouard et al. (2012) using 4-methylumbelliferyl-β-D-glucuronide (4-MUG, Sigma) as substrate. Immature seeds of stages S2, S3, and S4 were cut longitudinally with a scalpel and the embryos were removed with a needle, GUS activities of seed coats and embryo were measured separately. Whole seeds were used for earliest stages (S0 and S1, embryo too small), and latest stage S5 (mature seeds).
Copyright and License information: ©2016 Fang, Ramsay, Renouard, Hano, Lamblin, Chabbert, Mesnard and Schneider.
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