Transient expression in Arabidopsis protoplasts

DB Deepak D. Bhandari
DL Dmitry Lapin
BK Barbara Kracher
PB Patrick von Born
JB Jaqueline Bautor
KN Karsten Niefind
JP Jane E. Parker
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Leaf mesophyll protoplasts were prepared from 4-week-old eds1-2 pad4-1 sag101-3 plants and transfected with plasmid DNA69. After transfection, protoplasts were incubated at room temperature under weak light (1.5 μE/m2s) for 16 h. Protoplasts were harvested and IPs performed as described below.

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