Rhodopsin-loaded vesicles (RLV’s) counting

RT Rajan Thakur
AP Aniruddha Panda
EC Elise Coessens
NR Nikita Raj
SY Shweta Yadav
SB Sruthi Balakrishnan
QZ Qifeng Zhang
PG Plamen Georgiev
BB Bishal Basak
RP Renu Pasricha
MW Michael JO Wakelam
NK Nicholas T Ktistakis
PR Padinjat Raghu
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Whole mount preparations of photoreceptors stained with anti-Rh1 were imaged on Olympus FV1000 confocal microscope using Plan-Apochromat 60X, NA 1.4 objective (Olympus). The RLV’s per ommatidium were counted manually across the Z-stacks using Image J software from NIH (Bethesda, MD, USA).

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