Whole mount preparations of photoreceptors stained with anti-Rh1 were imaged on Olympus FV1000 confocal microscope using Plan-Apochromat 60X, NA 1.4 objective (Olympus). The RLV’s per ommatidium were counted manually across the Z-stacks using Image J software from NIH (Bethesda, MD, USA).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.