Scanning electron microscope

Evaluating the morphology of polymeric scaffolds in terms of pore size, characterizing fiber diameter, and being interconnected was performed by scanning electron microscopy (SEM, with Philips XI30).

To measure porosity, SEM images as inputs in image processing were used in MATLAB 7.11.0.584 software (developed by MathWorks, Natick, Massachusetts, United States of America, since 1984).[9] The MATLAB program was set to measure porosity. The diameters of micro and nanofibers were measured by ImageJ software on the SEM images. For each sample to measure fiber diameter, thirty nanofibers of fabricated web were selected and analyzed.