2.9. Cell culture

XC Xingxing Chen
WG Wenhua Ge
JH Jie Hu
TD Tiancheng Dong
HY Hui Yao
LC Lingzhi Chen
BG Bin Geng
HZ Hao Zhou
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We purchased primary rat cardiac microvascular endothelial cells (CMECs), cardiac fibroblasts (CFs), cardiomyocytes (CMs) from the cell bank of the Chinese Academy of Science. Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Lonza) with 10% foetal bovine serum (FBS; Gibco), 100 U/mL penicillin (Gibco) and 100 mg/mL streptomycin (Gibco). All cells were maintained at 37°C in 5% CO2 incubator.

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