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Superoxide dismutase activity (SOD) determination
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The injured-side cortices from rats were isolated at 6 hours post-TBI. The cortex tissue was placed into a 1.5 mL centrifuge tube. 250 μL of RIPA buffer was added with protease inhibitors. Homogenate was then centrifuged at 11,000 × g for 10 minutes at 4°C. SOD activity of the cortex tissue was determined using Xanthine Oxidase Assay Kits (Nanjing Jiancheng Bioengineering Institute) and expressed as units per milligram of protein.
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