Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

List of antibodies and growth factors

FC Federico Corti
YW Yingdi Wang
JR John M. Rhodes
DA Deepak Atri
SA Stephanie Archer-Hartmann
JZ Jiasheng Zhang
ZZ Zhen W. Zhuang
DC Dongying Chen
TW Tianyun Wang
ZW Zhirui Wang
PA Parastoo Azadi
MS Michael Simons
request Request a Protocol
ask Ask a question
Favorite

List of antibodies with application and dilutions (immunohistochemistry (IHC); western blot (WB)): pAKT T308 (WB 1:1000, Cell Signaling #4056), Total AKT (WB 1:1000, Cell Signaling # 4691), ERG (IHC 1:100, Abcam #ab92513), pERK (WB 1:1000, Cell Signaling #9106), total ERK (1:2000, Cell Signaling #9102, WB), FGFR1 (Cell signaling #9740, WB 1:1000), Flag-tag (WB 1:500, Sigma-Aldrich F1804), HA-tag (WB 1:1000, Cell signaling #3724), NRP1 (Cell signaling #3725, WB, 1:1000), pIntegrin (Itg)-β3 Y759 (WB 1:200, Santa Cruz #sc136458), total Integrin-β3 (WB 1:1000, Cell Signaling #13166) PECAM1 (IHC 1:200, BD Pharmingen #553370), Sdc2-mouse (IHC 1:200, LSBIO #LS-B2981), Sdc2-mouse (WB 1:200, R&D #AF6585—polyclonal raised against mouse Sdc2 ED), Sdc2-human (WB 1:200, R&D # AF2965, polyclonal raised against mouse human ED), Sdc4 (WB 1:500, Abcam #ab24511—monoclonal against C-terminal domain), pSRC Y416 (WB 1:1000, Cell Signaling #6943), Total Src (WB 1:1000, Cell Signaling #2109), pVEGFR2 Y1175 (WB 1:1000, Cell Signaling #2478), total VEGFR2 (WB 1:1000, Cell Signaling #2479), VE-Cad (WB 1:200, Santa Cruz #sc-6458 C-19 clone), GFP (Santa Cruz #sc-9996, WB, 1:200), and Syntenin (WB 1:500, Abcam #ab19903). List Growth factors: VEGFA165 (R&D #293-VE-010), VEGFA121 (R&D #4644-VS-010), FGF2 (R&D #233-FB-025).

Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A