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2.4. Cell Viability (MTT Assay)

HM Helder Massaro
LZ Lígia F. A. Zambelli
AB Auriléia A. de Britto
RV Rodolfo P. Vieira
AL Ana P. Ligeiro-de-Oliveira
DA Denise C. Andia
MO Marcelo T. Oliveira
AL Adriano F. Lima
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For the experimental and control groups (n = 6), the cell viability was evaluated using MTT assay by measuring the succinic dehydrogenase (SDH) activity, evaluating the mitochondrial activity of cells by the methyl tetrazolium reaction. The assays were made in triplicate at three independent experiments. As previously described, the cell metabolism was evaluated 6 and 24 h after exposure to the eluates [26]. The cell viability was obtained in absorbance of SDH activity and transformed in percentages considering the control group (DMEM) as 100%.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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