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Total RNA was extracted from the whole bodies of P. xylostella second instar larvae using Trizol reagent (Invitrogen/Life Technologies, Paisley, UK) as per manufacturer's instructions and then quantitated by calculating the absorbance ratio and by agarose gel electrophoresis. First strand cDNA was synthesized from 2 μg of RNA with the RevertAid First Strand cDNA Synthesis Kit K1622 (Thermo Scientific, US) by following the manufacturer's protocol.
Copyright and License information: ©2016 Fang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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