2.5. Transwell cell migration and invasion assay

At 24 hours after transfection, overexpression rates of miRNA‐150, and lncRNA CASC11 both reached 200%. Cell migration and invasion abilities were then tested at 24 hours after transfection. Cell suspensions (3 × 10⁴/mL) were prepared using serum‐free medium and transferred to the upper chamber with 100 µL in each well. The lower chamber was filled with ATCC‐formulated Eagle Minimum Essential Medium with FBS added to a final concentration of 20%. Cells were cultivated in an incubator (37°C; 5% CO₂) for 24 hours. After that, the upper chamber was collected and membranes were stained with 0.5% crystal violet (Sigma‐Aldrich) at room temperature for 20 minutes. The upper chamber was precoated with Matrigel (356234; Millipore, Bedford, MA) before the invasion assay. Stained cells were counted under an optical microscope (Olympus, Tokyo, Japan). Cell migration and invasion were normalized to cell proliferation.