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To observe the autophagosomes, NP Cells were transfected with a GFP-LC3-expressing adenovirus for 24 h. Subsequently, the transfected cells were used for the next experiments. The autophagosomes were observed through laser confocal microscopy and the level of autophagy was evaluated with the number of green fluorescent puncta of autophagosomes.
Copyright and License information: ©2019 Yi et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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